The Third Generation Laser Speckle Imaging System RFLSI-ZW is Coming Out Soon

   |  May 10, 2022

RWD will launch the third-generation Laser Speckle Imaging System (RFLSI-ZW) in 2022, maintaining its continuous influence and supporting role in the field of microcirculation research.
The eye-catching upgrades of the third-generation RFLSI-ZW include:

Highlight 1: The TR-LSCI technology laser transmission module is built into the product. Not only are the imaging contrast and detection depth better, but the testing of vascular functional information is also supported.

Highlight 2: The change indicators for oxyhemoglobin (oxyHb) and deoxyhemoglobin (deoxyHb) can be tested as never before.

Highlight 3: The expanded detection area makes the experience of power zoom and auto focus better.

blood flow imaging
blood flow imaging

Compared with RFLSI Ⅲ, the detection area of RFLSI-ZW has been increased by more than 8 times.

  • Unexampled Advantages of TR-LSCI Technology Laser Transmission Module

For traditional LSCI technology, light must penetrate the tissue layers (e.g., skin & skull) above blood vessels, where the light signal would be continuously attenuated. With better imaging contrast and resolution than conventional LSCI, Transmission detection laser speckle contrast imaging (TR-LSCI) can even support functional imaging of human finger joints in the absence of other complementary means.

In December 2021, Zhu Dan’s Team, from the Key Laboratory of Center for Biomedical Photonics, Huazhong University of Science and Technology, published “Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration” in Light[1]: Science & Applications (IF=14.098), elaborating on the advantages of TR-LSCI technology over LSCI technology: Unexampled Advantages:

  1. The comparison between TR-LSCI and LSCI tissue model imaging revealed that TR-LSCI surpasses traditional LSCI in terms of depth, and the imaging performance of TR-LSCI will not decrease significantly with the progressively deeper signal.
  2. The comparison of rheographies at in-vivo parts of laboratory animals manifested that the SBR of TR-LSCI is higher than that of traditional LSCI in each ROI, and TR-LSCI is easier to capture a more comprehensive rheography. It is easy to observe the vessel information at different depths of the sample through TR-LSCI.
  3. The comparison of blood flow sensitivity indicated that TR-LSCI has unparalleled advantages not only in deep tissue blood flow mapping, but also in the sensitivity of low-velocity blood flow changes.
  4. TR-LSCI monitored ACh-induced vascular functional responses in mouse’s hindlimbs, providing the data on individual vascular structures and functions.
  5. Blood flow imaging in the dorsal vessels of the palm cannot be obtained by traditional LSCI, but blood flow in the vessels at the corresponding location can be clearly visualized by TR-LSCI.
  • Building on blood flow indicators, the change indicators for oxyhemoglobin (oxyHb) and deoxyhemoglobin (deoxyHb) are newly added

As early as 2013, André Steimers[2], a German scientist, together with his team members, published an article—”Simultaneous Imaging of Cortical Blood Flow and Haemoglobin Concentration with LASCA and RGB Reflectometry”. In this article, they proposed the possibility of simultaneous blood flow and blood-oxygen functional imaging with LASCA and RGB reflectometry. The constructed system was designated to test the system sensitivity by observing changes in hemoglobin oxygenation and blood flow in rats in response to ischemic stroke, hypercapnia, hyperoxia, hypoxia, cortical diffusion inhibition, and cortical activation after forepaw stimulation.

 simultaneous blood flow and blood-oxygen functional imaging

The RWD Laser Speckle Imaging System will meet sundry complex microcirculation scenarios in an even better way with further improvement in its performance.
Stay tuned for the upcoming third-generation Laser Speckle Imaging System is about to be launched.

 Laser Speckle Imaging System RFLSI-ZW

[1]Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration[J]. Light: Science & Applications.

[2] Steimers A , Gramer M , Takagaki M , et al. Simultaneous imaging of cortical blood flow and haemoglobin concentration with LASCA and RGB reflectometry.[J]. Oxygen Transport to Tissue XXXIII, 2013, 789:427-433.

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