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  • Tissue-Enzymatic-Dissociation-Kit

High Activity FFPE Tissue Enzymatic Digestion Kit-25T

High Activity FFPE Tissue Enzymatic Digestion Kit-is designed to gently, rapidly, and efficiently prepare FFPE (formalin-fixed paraffin-embedded) tissue sectioninto single-cell suspensions. The optimized protocol maximizes the yield of viable single-cell samples while preserving critical antigenic

epitopes, ensuring the integrity of cellular structures. The resulting single-cell suspensions are suitable for flow cytometry analysis, as well as other genetic or proteomic studies.

Cat No: DHFTE-2517

Number of reactions: 25T

Technical Specifications

  • Data Support
  • Q&A
  • Related Resources

High-Yield Single-Cell Dissociation of Breast Cancer FFPE Tissues

Breast cancer FFPE tissues were processed using the RWD FFPE Tissue Enzymatic Digestion Kit, Single-Cell Suspension Dissociator, and tissue processing tubes, following the standardized protocol to obtain single-cell suspensions. The analysis demonstrated a nucleated cell rate of 51% with a total cell yield reaching 5×10⁴ cells/mg.

 

Q1:What are the storage conditions for the tissue enzymatic digestion kit?
A1:Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions.

Q2:What are the pre-treatment precautions for tissue samples?
A2:During the sampling process, carefully remove the blood vessels and residual blood clots, and rinse the tissue thoroughly.

Q3:What are the types of human tumor tissue to be processed?
A3:Tumor samples obtained directly from patients, cultured samples of human tumor tissue grown on nude mice, and cultured samples of human tumor cell lines on nude mice.

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