The fiber photometry system records changes in the fluorescence intensity of neurons in a specific brain area to reflect neuronal population activity. In the study of neural circuits, the fiber photometry system can perform long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal behavior.
- Main Applications:
Ca2 + and neurotransmitter signal detection
Study the function of neural circuits
Explore the mechanisms of neurological diseases
Develop new fluorescent sensor probe
Experiment of optical principle
Three kinds of excitation light sources, that is 410nm、470nm and 560nm, are respectively used for excitation
of reference, green fluorescence and red fluorescence;
Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain
Dual highly sensitive detectors enabling independent and sequential detection to avoid interference of
fluorescence excitation and detection, acquiring more accurate signal;
Professional acquisition and analysis softwares are flexible and easy to operate with data processing functions
available. No matlab programming is required;
Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon
trigger, delayed acquisition and timing acquisition;
Live display of DeltaF/F acquisition to check scale of signal changes during acquisition;
Customized adjustment of output signal parameter, easily trigger and control external excitation equipment
to achieve closed-cycle control of excitation and recording;
Compatible with optogenetics for recording and stimulation at the same site.
Stable Data Acquisition
- Successful Case
Journal：Cell Stem Cell
Demand of the Product：We recorded calcium dynamics in DG-projecting SuM neurons by fiber photometry. In vivo recordings were carried out in an
open-top HC (21.6×17.8×12.7 cm3) or EE (45×25× 20 cm3) containing toys, colorful balls, domes or tunnels and food, for 10min.
Cite Reference：Li, YD., Luo, YJ., Chen, ZK. et al. Hypothalamic modulation of adult hippocampal neurogenesis in mice confers activity-dependent regulation of memory and anxiety-like behavior. Nat Neurosci 25, 630–645 (2022). https://doi.org/10.1038/s41593-022-01065-x
Demand of the Product：We performed fiber photometry to record calcium activity of CA3 and CA1 pyramidal neurons labeled with GCaMP6f upon chemogenetic activation of SuM-enhanced ABNs by intraperitoneal CNO injection.
Cite Reference：Li YD, Luo YJ, Xie L, Tart DS, Sheehy RN, Zhang L, Coleman LG Jr, Chen X, Song J. Activation of hypothalamic-enhanced adult-born neurons restores cognitive and affective function in Alzheimer’s disease. Cell Stem Cell. 2023 Apr 6;30(4):415-432.e6. doi: 10.1016/j.stem.2023.02.006