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Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability and cell yeid-Preserving cell surface epitopes leads to no effect for downstream experiments.

 

Application:

Dissociative tissue types: people, mice or rats all kinds of organizations.
Downstream application experiments: primary cell culture, flow analysis, single cell sequencing, etc.

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Technical Principles

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Easy-to-follow procedure

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Target Extracellular Matrix

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Specific Formation

1.Simple and easy to follow experimental procedure

2.Dissociating cells against the extracellular matrix with maximum retention of cell surface epitopes.

3.Optimized enzymatic formulation ensure highly active single-cell suspensions.

Specification parameters

Cat No.Product nameNumber of reactions
DHTE-5001High Activity Tumor Tissue Enzymatic Digestion Kit(Mouse)50TLearn more
DHNBE-5002High Activity Neonatal Brain Enzymatic Digestion Kit(Mouse and Rat)50TLearn more
DHABE-5003High Activity Adult Brain Enzymatic Digestion Kit(Mouse and Rat)50TLearn more
DHGT-5004High Activity General Tissue Enzymatic Digestion Kit(Mouse)50TLearn more
DHTEH-2505High Activity Tumor Tissue Enzymatic Digestion Kit(Human)25TLearn more
DHIE-5007High Activity Intestine Tissue Enzymatic Digestion Kit(Mouse)50TLearn more
DHBTE-2508High Activity Brain Tumor Enzymatic Digestion Kit25TLearn more
DHWSE-2509High Activity Whole Skin Enzymatic Digestion Kit(Mouse)25TLearn more
DHAE-5010High Activity Adipose Tissue Enzymatic Digestion Kit(Mouse and Rat)50TLearn more
DHDR-5006High Efficiency Debris Removal Kit50TLearn more

Q&A

Q1:What is the value experience of using the RWD enzymatic digestion kit for users? +

The RWD enzymatic digestion kit is a device that prepares high-activity single-cell suspension and tissue homogenate. It offers a valuable experience for users by providing convenience, efficiency, and a wide range of applications. It can process many types of tissue samples, improve the efficiency of the experiment, provide the optimal enzyme solution formula, reduce tedious repetitive operations, and ensure the repeatability of experiments. It achieves more than 90% of high cell viability and high single-cell suspension yield, with moderate digestion and maximal preservation of cell surface antigens.

Q2:What is the difference between the RWD enzymatic digestion kit and the customer-made enzyme digestion kit?+

The RWD enzymatic digestion kit is a standardized enzyme digestion kit that has been tested and optimized for the appropriate enzyme ratio and concentration.

Q3:What are the storage conditions for the tissue enzymatic digestion kit? +

Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions. The debris removal reagent in the adult brain kit must not be frozen.

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