High Activity Neonatal Mouse And Rat Heart Enzymatic Digestion Kit

High Activity Neonatal Mouse and Rat Heart Enzymatic Digestion Kit can prepare heart tissue of neonatal mice or rats (P0-P3) into single cell suspension gently, quickly, and efficiently. This optimized protocol can help obtain as many single cell samples with high cell viability as possible, while preserving the important surface epitopes of cells. The obtained single cell suspension can be applied in downstream experiments such as cell culture or sorting of cardionnyocyte, fibroblast and endothelial cell, flowcytometry analysis, and single cell sequencing.

Cat No: DHFTE-2518

Number of reactions: 25T

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High-Viability Single-Cell Preparation from C57 Mouse Heart Tissues

C57 mouse heart tissues were processed using the High Activity Neonatal Mouse And Rat Heart Enzymatic Digestion Kit, Single-Cell Suspension Dissociator, and tissue processing tubes, following the standardized protocol to obtain single-cell suspensions. The quality control analysis demonstrated that the cell viability consistently exceeded 90%.

Q1:What are the storage conditions for the tissue enzymatic digestion kit?
A1:Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions.

Q2:What are the pre-treatment precautions for tissue samples?
A2:During the sampling process, carefully remove the blood vessels and residual blood clots, and rinse the tissue thoroughly.

Q3:What are the types of human tumor tissue to be processed?
A3:Tumor samples obtained directly from patients, cultured samples of human tumor tissue grown on nude mice, and cultured samples of human tumor cell lines on nude mice.

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