Precise Annealing Temperature Control with Gradient Thermal Cycler
| August 8, 2022
The polymerase chain reaction (PCR) is fundamental to many of the procedures used in molecular experiments. During a single cycle of the PCR process, the annealing temperature is a key point to decide if the small samples could be amplified to a large amount.
In the annealing process, the temperature should be as low as possible to ensure effective amplification of primers and target sequences, meanwhile, the temperature should be high enough to reduce non-specific binding.
In short, a proper temperature strongly determines the efficiency and specificity of the results.
A common thermal cycler VS A gradient thermal cycler
A common thermal cycler—only a specific annealing temperature could be set during the process.
A gradient thermal cycler—could not only be used as a common thermal cycler but also set different temperature gradients (12 groups of gradient function is common). Optimizing the annealing temperature during PCR procedures enables success with a maximum yield of targets and specificity.
How about RWD gradient thermal cycler?
RWD self-developed M2-96G gradient thermal cycler with 96-well provides 12 groups of linear temperature gradient function, helping to realize at one time to optimize PCR protocol. Meanwhile, different types of PCR experiments such as Touchdown PCR and Long PCR can be operated as well. With precise temperature control and an intelligent user operating system, M2-96G could achieve rapid and specific amplification of target DNA fragments.
1) 12 linear gradients in the same run allow to optimize the ideal annealing temperature 2) Personalized account management system provides organized programs and unwanted changes 3) 7 inch touch screen enables intuitive and quick programming 4) Capable for PCR experiments and constant temperature incubation function
It is widely used in molecular biology, microbiology, genetics, cell biology, food science and agronomy to carry out experimental research such as molecular cloning, gene expression analysis, genotype identification, sequencing, and pathogenic microorganism analysis.