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  • cell counting and analysis
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C100/C100-SE Automated Cell Counter

A Real Time-Saver Speeds up Your Research.

RWD automated cell counters provide two models to meet different customers’ needs in BF&FL cell counting and analysis in 9s. Combined with highly intelligent software and excellent microscopy optics structure, the counters could liberate researchers from the heavy work of daily cell counting.


“So far, I have only heard good things about this Cell Counter, so very happy with it. ”

— —Ph.D Claudio David, University of Heidelberg


“The features and general technical standards are excellent. Speed, touchscreen, smooth loading of samples to the slide are all excellent features available in RWD C100 Automated Cell Counter.”

— —Preyesh Stephen, Queens University, Ontario Canada


“Dual fluorescence channels make counting applications more extensive.”

— —Chinese Academy of Sciences

Technical Specifications

  • Overview
  • Advantages
  • Specifications
  • Order information

The automated cell counter can identify both live and dead cells. Information such as cell counts, viability and diameter can be obtained with just one click. With fluorescence cube, C100 can display images in BF and FL channels simultaneously and present cell counts and cell morphology. It is an optimal device to analyze cell viability and transfection efficiency in immunology and vaccine development, cell therapy, tumor research, stem cell, metabolism research, and others.


Tumor Research ⸺ Passage cell line, pre-detection of apoptosis

Immunological research ⸺ PBMCs, lymphocytes

Cell Biology Research ⸺ Primary Cells

Cell quality control ⸺ stem cells, CIK

Cell expression level detection ⸺ fluorescent protein, transfection efficiency detection

Smart cell recognition

Clustered cells can be identified and counted separately. In trypan blue mode, the counting result will show the proportion of live cells and dead cells.

The results of HEK293 cell line and mouse primary macrophage count showed:

C100 counter has a smaller standard deviation of multiple counts.

The actual measured value and the theoretical value have a slight deviation in a great linear relationship.

The CV value of manual counting can reach more than 10%, while the CV value of the C100 counting result is much lower than 5%.

Fast cell counting in 9 seconds

Compared to the hemocytometer, it can save you more than 24hrs within a month when counting 10 samples per day

It takes about 5 minutes to use a hemocytometer for manual cell counting, while C100-SE/C100 only takes 9s. Counting ten samples per day can save more than 24 hours a month.


User-friendly operation

Touchscreen with built-in user guide interfaces ensures easy operation.


Compact design

Small size fits into different placements.

Removable modular FL cubes allow easy switches among various scenes.

Parameters Description
Model C100-SE C100
Optional fluorescence application N/A


(Ex :375/28nm ;Em:460/50nm)

(Ex :480/30nm ;Em:535/40nm)

(Ex :540/25nm ;Em:620/60nm )

Amplification Factor 2.5×, 5.0 Megapixel
Focus Method Manual focusing & Autofocusing
Counting Area 2.15 mm×1.62 mm
Cell Type Cell lines, stem cells, primary cells, pollens, beer yeast
Cell Size Range 4-60 μm (Optimal: 7-60 μm)
Cell Concentration Range 10^4-10^7 cells/mL
Cell counting Time Less than 9s
Historical Data Storage 1000 counting reports and images at most
Languages Chinese and English
Dimensions 212 mm(W)* 264 mm(H)* 165 mm (D)
Weight ~3.0kg ~3.2 kg


FL Cube Excitation Emission General Dyes
FL Cube-01 375/28nm 460/50nm DAPI,Hoechst,BFP
FL Cube-02 480/30nm 535/40nm Acridine Orange(AO),Calcein-AM,Alexa fluor 488,SYBR Green,FITC,GFP
FL Cube-03 540/25nm 620/60nm Propidium Iodide(PI),DsRed,dTomato,RFP
Cat No. Product Description
C100 Automated Cell Counter (BF + FL)
C100-SE Automated Cell Counter (BF)
FL Cube-01 (Ex :375/28nm ;Em:460/50nm)
FL Cube-02 (Ex :480/30nm ;Em:535/40nm)
FL Cube-03 (Ex :540/25nm ;Em:620/60nm )
DS-50 Disposable Slide (50 pcs/box)


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