Flow cytometry analysis
Immune cell subsets can be detected by labeling cell surface antigens with CD45, CD207, CD11b, CD11c.
Single cell sequencing
All cell populations were able to be mapped with highly similar proportions and low mitochondrial gene percentages compared to published literature data.
It is confirmation that skin tissue samples dissociated from the RWD Tissue Enzymatic Dissociation Kit can be used directly for single cell sequencing.
Q1:What are the storage conditions for the tissue enzymatic digestion kit?
A1:Different types of enzymes are contained in different kits, and they should be stored in refrigerators at 4°C or -20°C according to the storage requirements on the instructions.
Q2:What are the pre-treatment precautions for tissue samples?
A2:During the sampling process, carefully remove the blood vessels and residual blood clots, and rinse the tissue thoroughly.
Q3:What are the specific parts of the skin tissue to be extracted?
A3:Mouse skin tissue from the back or ear.