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RWD Fiber Photometry System
R820 Tricolor Multichannel Fiber Photometry
fiber photometry calcium imaging
Multi Fiber Photometry System
Fiber Photometry System
fiber photometry principle
RWD Fiber Photometry System
R820 Tricolor Multichannel Fiber Photometry
fiber photometry calcium imaging
Multi Fiber Photometry System
Fiber Photometry System
fiber photometry principle

R820 Tricolor Multichannel Fiber Photometry System

R820 fiber photometry system is featured with an integrated lightweight body and richer software, can record red&green fluorescence signal, such as GCaMP, RCaMP, dLight, and jrGECO1a, while a unique 410nm LED is used to acquire control signals and exclude noise. It can support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations. And also it is easy connected with other equipment such as optogenetics and electrophysiology.

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Overview

Fiber photometry calcium imaging is becoming increasingly popular in neuroscience research and, based on its advantages, is now an indispensable tool for the real-time detection of neural signals.
R810 Dual Color Multichannel Fiber Photometry System has two excitation light sources (410nm and 470nm). The 410 nm can be used to reflect the background noise signal, thus ensuring the acquisition of true fluorescence data.
R820 Tri-Color Multichannel Fiber Photometry System has three excitation light sources (410nm, 470nm and 560nm).The system can record signal of green fluorescence indicator like GCaMP and dLight or neurotransmitter probe and red fluorescence indicator like RCaMP, jrGECO1a or neurotransmitter probe.

Main Applications:

  • Ca2 + and neurotransmitter signal detection
  • Study the function of neural circuits
  • Explore the mechanisms of neurological diseases
  • Develop new fluorescent sensor probe
  • Experiment of optical principle

 

Principle:

Fiber photometry is a technology to detecting the activity of neurons in the brain nucleus of freely moving animals. It sums up the overall fluorescence of neurons expressing a genetically encoded calcium indicator(GECI) or neurotransmitter probes.
Fiber photometry calcium imaging has been in development for nearly a decade and has been highly rated by many laboratories. It can be used to explore the regulatory mechanisms behind animal behavior.

In fiber photometry, data are collected by analyzing the fluorescence change (ΔF) relative to the initial baseline fluorescence (F) and observing the signal change corresponding to the calcium transient (ΔF/F). These metrics are usually based on fluorophores such as GFP, RFP, tdTomato, mCherry, etc., of which GCaMP is the most common example.

In neurons, calcium ions regulate several important processes, including neurotransmitter release and membrane excitability. Fiber photometry calcium imaging can provide critical insight into calcium dynamics, as GCaMP fluoresces only when bound to calcium ions. Based on similar principles, fiber photometry systems can also detect changes in the concentration of neurotransmitters.
(Neurotransmitter probes – cpEGFP is embedded in a specific neurotransmitter receptor, and the binding of the receptor to the neurotransmitter triggers a conformational change in the receptor to convert to a fluorescent signal, and this genetically encodable probe is expressed in cells or mouse brain by viral injection, transfection, and other technical means.)

Advantages

Integrates data acquisition, analysis & plotting

Bonsai software and MATLAB programming are not required. Data analysis includes data clipping, bleaching correction, smoothing, movement correction, event heat map, peak statistics, and area under curve and heat map of behavior trajectory.

Multiple Behavioral Events Synchronize & Mark

The software can synchronize and mark multiple special behavioral events or external input signals during the experiment.

More Accurate Signal, Avoid Interference

Dual highly sensitive detectors enabling independent and sequential detection to avoid interference of fluorescence excitation and detection, acquiring more accurate signal. The 410nm light source can be used to reflect the background noise signal, thus ensuring the acquisition of true fluorescence data.

Easy Connection to Other Equipment

4-input and 4-output interface for easy connection with other equipment such as optogenetics and electrophysiology for the closed-loop study of stimulation and recording.

9 Channels Support

Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations

Specifications

Wavelength of excitation light R820 : 410nm  470nm   560nmR810 : 410nm  470nm
Power Min 0µW, Max≥100µW, adjustable with an accuracy of 0.1µW
Number of channels 9
Frame rate of fluorescent sampling Max 250fps
Digital signal interface 4Input 4Output
Signal output Output frequency 0-500Hz, adjustable output pulse width and duration
Marking Manual marking (10), automatic marking (4), ROI marking (9)
Behavior camera 1920*1080(30fps) 1280*720(60fps) Switchable among multiple frame rates of resolution

 

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