The rodent limb ischemia preparation is a wild used model of peripheral arterial disease (PAD). The model uses a Laser speckle imaging system to real-time measure blood perfusion (PU) in limbs, and we can get imaging of blood vascular to distribute. When we draw the ROIs (region of interest), the software enables the measurement of the blood flow. As we can see, the difference in PU between the ischemic versus and non-ischemic limb (Fig1). And we can do some research about the “Reperfusions” which can measure the function of drugs in the PAD. And if you want to have a free demo of the laser speckle, you can click here.
Equipment recommendations
The Laser Speckle Contrast Imaging System (RFLSI Ⅲ) offers the highest spatial resolution (2048 × 2048) and can be used to assess reperfusion in the pads of the feet(Xu et al., 2020). RFLSI Ⅲ is the ideal equipment for recording the process of reperfusions and vessel formation.

Fig 1. Right limb: ischemic versus, left limb: non-ischemic limb.
Reference:
Xu, X., Wang, W., Wang, Z., Lv, J., Xu, X., Xu, J., Yang, J., Zhu, X., Lu, Y., Duan, W., et al. (2020). DW14006 as a Direct AMPKalpha Activator Ameliorates Diabetic Peripheral Neuropathy in Mice. Diabetes 69, 1974-1988.