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RFLSI Ⅲ Laser Speckle Contrast Imaging System

Based on the Laser Speckle Contrast Imaging(LSCI) technology, RFLSI Ⅲ provides real-time and full-field blood flow monitoring and recording.
In non-contact way, reliable quantified data and high definition images could be obtained for various of pre-clinical research. It can be used to record the microcirculation of any exposed organ, such as the cortex, mesentery, lower limbs, and spinal cord.

Apply to related research topics:

  • Cerebral Cortex Blood Perfusion Monitoring
  • MCA-Occlusion Assessment
  • Cortical Spreading Depression Observation
  • Hindlimb Ischaemia Research
  • Skin Burn/Skin Flap Transplantation Research
  • Organ Microcirculation Observation


Brain Injury:

“What do you think of this image, It’s that clear enough? Yes, it’s very very very nice and very impressive”
–Dr. Amar Abdullah, Ph.D.  丨 Faculty of Medicine, Dentistry and Health Sciences


Stroke & Vascular Function:

“We can use the Laser Speckle Imaging System to observe the 5th branch of Mesentery.”
Yi-Je (Jay) Chen, 丨D.V.M., Ph.D. UC Davis


Cognitive Disorder:

“This is the sharpest speckle device I’ve ever seen, this is what I expected.”
–Lei P, Professor丨West China Second University Hospital

Technical Specifications

  • Key Features
  • References
  • Specifications
  • Operation &Installation
  • Configuration

It can be used to measure the microcirculation of any exposed superficial vessels, such as the cortex, mesentery, lower limbs, and spinal cord

1. Non-invasive, real-time, full-field imaging with high-definition; no need for scanning, no need of contact, no need of contrast for imaging

2. 12* Optical lens, autofocus, electrical zoom, which allow researchers to adjust the image area as they need. 12X Optical Zoom Lens is widely used in the vascular microcirculation of rodents

3. Built-in Basler camera with high-performance, resolution up to 2048*2048 pixels, and shooting speed up to 120 frames per second, resulting in higher definition and faster imaging.

4. Thorlabs laser is used to output laser with good coherence to ensure the stability of baseline data and obtain the fluctuation data of real blood perfusion.

5. Blood flowmeter (the volume of blood perfusion expressed as conventional unit PU) makes it easier to observe the relative changes in the velocity of blood flow by combining image data with perfusion data

6. The global PU value is adopted to quantify the values of blood perfusion and obtain the objective data changes in the velocity of blood flow.

7. Combining the image data and perfusion data, the velocity of blood flow can be observed by the pictures, and the relative velocity of blood flow can be judged by different colors. (Perfusion Unit)

8. The dual 3.0 USB interface is adopted to make data transmission faster and obtain data of higher frame rate and higher resolution

9. Hardware structures including instruction laser and Cardan gimbals and humanized software operation mode make the whole system easy to use

10. RWD RFLSI Ⅲ has been used in more than 100 colleges, universities, and research institutes in the world

RFLSI Ⅲ Parameters
Scale 0 – 5000PU
Imaging acquisition rate Up to 120 FPS
Working distance 10 – 35 cm
Image size 1.57 × 1.57mm – 90 × 90mm
Image camera pixels 4.2 million(2048 × 2048)
Max resolution Up to 3μm/pixel
Zoom range 12× optical zoom lens with auto-focus
Wavelength 785nm
Name Type Notes
Laser speckle imaging system RFLSI Ⅲ Mainframe; Computer; Holder; Software;

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