As an important kinase regulating energy homeostasis, AMPK is one of the central regulators of eukaryotic cell and organism metabolism, responsible for regulating the input and output of cell capacity and maintaining the smooth operation of cell physiological activities. AMPK is also a key protein involved in multiple signaling pathways. Once activated, AMPK mainly regulates four major categories of metabolism in mammals: protein metabolism, lipid metabolism, carbohydrate metabolism, autophagy, and mitochondrial homeostasis, almost covering the entire physiological metabolic activities of living organisms.
AMP-activated protein kinase (AMPK) senses oxidative stress and mitochondrial function playing a central role in the regulation of Diabetic peripheral neuropathy (DPN).
DW14006 as a direct AMPKα activator efficiently ameliorated DPN in both streptozotocin (STZ)-induced type 1 and BKS db/db type 2 diabetic mice.
Demands of the Research
The real-time regional velocity and distribution of blood flow and perfused blood vessels of the sciatic nerve and foot pads need to be detected.
Along with Western blot results, figure out the signaling pathway from DW14006-AMPK-NAD+-SIRT1-PGC1-Mito biogenesis Oxidative metabolism to Diabetic Neuropathy.